and innovative component of the
HTAS is the tissue/membrane
holding “cartridge”. These cartridges are designed to hold, seal and, in
certain applications, cut live tissue or artificial
membranes for insertion into HTAS system.
two types of cartridges are available to accommodate both the flat "coin"
sample of a tissue or artificial membrane or the tubular sample of tissue
These cartridges have dual chamber
assemblies with the supplying chamber and the receiver chamber interfaced
with each other through the membrane (tissue sample). Tissue samples are
inserted into cartridges, sealed and installed into the holding frame of the
HTAS in an automated fashion.
chambers of the cartridge have been designed to hermetically accommodate
opposing spring loaded fittings, which introduce all conduits and testing
electrodes and serve as a holding and sealing clamp. Fluidic conduits have
a multi-lumen design to accommodate parallel lines carrying buffers,
compounds, gases and sample acquisition.
frame holding an array of the cartridge chamber assemblies is installed into
an incubation chamber, which will accommodate all the spring-loaded feeding
fittings and provides a controllable environment for ADMET studies. The
chamber assemblies are in constant fluid communication with buffer, gas,
compound and sample reservoirs located outside the incubation chamber. The
array of chamber assemblies can rotate within the incubation chamber along
its long axis to provide horizontal or vertical positioning of the
membranes, and gravitational and centrifugal leveling of compounds to
enhance liquid to membrane interactions.