IR Concentrating Plates

 

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Two types of IR radiation enhancing plates are introduced:

Plates with semi-spherical IR reflecting wells and plates with parabolic reflecting wells

Plates were developed and tested to serve as reaction chambers and sensitivity enhancers (concentrators) of thermal activity (IR emission). Both types of plates are molded from thermally insulating plastic (which minimizes external thermal noise) and coated with an IR reflecting layer of gold.

Semi-spherical well plates:

 

When two semi spherical plates are sealed against each other, an array of IR radiation integrating spheres is produced.

The bottom plate usually serves as a reaction chamber, while the top matching plate accommodates an array of sensors. These sensors provide an integrated signal proportional to the internally generated thermal IR emission. Various sensors can be embedded into the top plate wells either directly, or linked via special IR transmitting optic fibers. Utilization of fiber-optic links provide the flexibility to position sensors in a remote environment for enhanced performance. (i.e. liquid nitrogen - for reduced thermal noise and increased sensitivity).

Example of large individual parabolic wells

Parabolic well plates:

Parabolic well plates have a slightly different IR monitoring characteristics. Because they concentrate and direct IR energy from it's focal point  upwards, it is possible to monitor the emission in two diffrent ways:

a) Using an mating opposing plate with sensors located also in focal point, providing signal amplification with minimal noise gain. 

b) Use a well developed hardware and software technology of FLIR cameras

In a typical thermal signature screening assay, a collection of individual compounds under investigation and reference blanks are dispensed into wells using the appropriate dispensing device. The common "binding ligand" is then dispensed by the area "shower" dispenser to trigger the simultaneous initiation of the binding process. This bottom (reaction) plate is covered immediately by the top sensing plate, which will begin to monitor the thermal signatures of each reaction and enable the comparative (ratiometric) analysis of activity from all wells. The data can then be integrated into a profiling database.

 

Techelan offers variety of techniques for individual dispensation of regents, as well as simultaneous dispensation of the

activating ligand in order to trigger the process simultaneously and to monitor the thermal dynamics of interaction

Individual aspiration/dispensation can be accomplished using TEDA type dispensers.

Simultaneous activation of the entire array can be performed using TEDA-300 and TEDA-400 type dispensers.