well plates have a slightly different IR monitoring characteristics. Because
they concentrate and
direct IR energy from it's focal point upwards, it is possible to
monitor the emission in two diffrent ways:
Using an mating opposing plate with sensors located also in focal point,
providing signal amplification with minimal noise gain.
Use a well developed hardware and software technology of FLIR cameras
typical thermal signature screening assay, a collection of individual
compounds under investigation and reference blanks are dispensed into wells
using the appropriate dispensing device. The common "binding ligand" is then
dispensed by the
"shower" dispenser to trigger the simultaneous initiation of the
binding process. This bottom (reaction) plate is covered immediately by the
top sensing plate, which will begin to monitor the thermal signatures of
each reaction and enable the comparative (ratiometric) analysis of activity
from all wells. The data can then be integrated into a profiling database.